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VirusTotal | As of April 5, 2009, VirusTotal will scan a file with 40 malware detection programs. For a list of the antivirus engines it uses see About VirusTotal. You can also send files to be scanned by email; see Sending files by email. Note that there's a 10MB file size limit, and if you forward a message with multiple attachments, VirusTotal will only check one. |
VirSCAN | Scans a file with 37 malware detection programs as of April 5, 2009. There is a 10 MB file size limit per file. |
Jotti | This is a non-profit service that will scan the uploaded file with 20 antivirus programs as of April 5, 2009. There is a 10 MB file size limit per file. |
There are also other sites where you can opt to have your entire system scanned for malware (see Free Online Virus Scanners)